Monosaccharide Indicator Standard Test
1. Test for Glucose: Mix 2 mL of a 2% glucose ( a monosaccharide) solution with 2 mL of Benedict's solution in a test tube. Heat 100 mL of water in a 250 mL beaker to 100 degrees C. Place the test tube in this water for 2 minutes. Record all the color changes the amount of time it takes for each change.
2. Test for water (negative control): 2 ml of deionized water with 2 mL of Benedict's Solution in a test tube. Heat 100 mL of water in a 250 mL beaker to 100 degrees C. Place the test tube in this water for 2 minutes. Record all the color changes the amount of time it takes for each change.
Starch Indicator Standard Test
1. Test for starch: Mix 2 mL of starch suspension(that has been mixed) with 0.25 mL of Lugol's iodine. Swirl to mix these. Don't heat. Record the color changes.
2. Test for water (negative control): Mix 2 mL of ionized water with 0.25 mL of Lugol's iodine. Swirl to mix these. Don't heat. Record the color changes.
Protein Indicator Test
Caution: Sodium Hydroxide (NaOH) is a strong, base and it is caustic; can burn. Follow lab safety procedures.
1. Test for protein: Place 2 mL of gelatin (protein) solution in a test tube. Add 0.5 mL of 10% NaOH and gently vortex to mix. Now add 0.25 mL of 5% copper sulfate (CuSO4) and mix. This NaOH and CuSO4 mixture is called Biuret reagent. Record the color change after 30 secs.
2. Place 2 mL of deionized water in a test tube. and 0.5 mL of 10% NaOH and gently vortex to mix. Now add 0.25 mL of 5% copper sulfate (CuSO4) and mix. Record the color change after 30 secs.
Sudan IV Indicator Test
1. Add 0.6 mL of Sudan to 2 mL of fat and then 2mL of water.
1. Test for Glucose: Mix 2 mL of a 2% glucose ( a monosaccharide) solution with 2 mL of Benedict's solution in a test tube. Heat 100 mL of water in a 250 mL beaker to 100 degrees C. Place the test tube in this water for 2 minutes. Record all the color changes the amount of time it takes for each change.
2. Test for water (negative control): 2 ml of deionized water with 2 mL of Benedict's Solution in a test tube. Heat 100 mL of water in a 250 mL beaker to 100 degrees C. Place the test tube in this water for 2 minutes. Record all the color changes the amount of time it takes for each change.
Starch Indicator Standard Test
1. Test for starch: Mix 2 mL of starch suspension(that has been mixed) with 0.25 mL of Lugol's iodine. Swirl to mix these. Don't heat. Record the color changes.
2. Test for water (negative control): Mix 2 mL of ionized water with 0.25 mL of Lugol's iodine. Swirl to mix these. Don't heat. Record the color changes.
Protein Indicator Test
Caution: Sodium Hydroxide (NaOH) is a strong, base and it is caustic; can burn. Follow lab safety procedures.
1. Test for protein: Place 2 mL of gelatin (protein) solution in a test tube. Add 0.5 mL of 10% NaOH and gently vortex to mix. Now add 0.25 mL of 5% copper sulfate (CuSO4) and mix. This NaOH and CuSO4 mixture is called Biuret reagent. Record the color change after 30 secs.
2. Place 2 mL of deionized water in a test tube. and 0.5 mL of 10% NaOH and gently vortex to mix. Now add 0.25 mL of 5% copper sulfate (CuSO4) and mix. Record the color change after 30 secs.
Sudan IV Indicator Test
1. Add 0.6 mL of Sudan to 2 mL of fat and then 2mL of water.